PART-III (PG LEVEL BIOLOGY)- 1. Environmental Biology: • Environmental Issues: Pollution, Green House Gases, Global Warming, Ozone Depletion, Deforestation, Soil Degradation. • Bio remediation, Waste water treatment, Solid waste management, Disposal of Bio medical waste. 2. Biotechnology and its Applications: • Microbial Culture: Nutritional Requirements & Culture Media, Sterilisation methods & Pure Culture Techniques. • r DNA technology. • Application of r DNA technology: DNA Finger printing, transgenic organisms, Gene therapy, Therapeutic Agents/Molecules- Monoclonal, Anti Bodies, Insulin, Growth Hormones. • Tissue Culture and its application: Plant and Animals 3. Molecular Biology: • Central Dogma of molecular Biology. • Chromosome - Structure & Organisation. • Gene Regulation in Eukaryotes and Prokaryotes. 4. Tools & Techniques: Basic Concepts: • Microscopy-Light and Electron microscopy. • Centrifugation • Electrophoresis • ELISA • Chromatography • Spectroscopy • In situ Localisation Techniques: FISH, GISH • DNA Sequencing 5. Biogeography and Wild Life Conservation: • Major terrestrial Biomes. • Biogeographical zones of India, Endemism, Hot spots, • Plant distribution with special reference to Rajasthan. • Wild life conservation/Management Strategies. • Concept of Protected areas. Biosphere reserves of India. Wild life sanctuaries, National Parks, Wetlands and Sacred grooves with special reference to Rajasthan.